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Expression of the gene for the multidrug resistance-associated protein in human prostate tissue

To characterize the clinical relevance of MRP gene in the chemoresistance of prostate carcinomas we determined the multidrug resistance-associated protein (MRP) expression in 30 samples from organ-confined prostate carcinoma, 9 samples from adjacent normal tissue and 4 hormone unresponsive cancers....

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Hauptverfasser: Schummer, Bernhard (VerfasserIn) , Siegsmund, Michael (VerfasserIn) , Steidler, Annette (VerfasserIn) , Toktomambetova, Lira (VerfasserIn) , Köhrmann, Kai-Uwe (VerfasserIn) , Alken, Peter (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: June 1999
In: Urological research
Year: 1999, Jahrgang: 27, Heft: 3, Pages: 164-168
ISSN:1434-0879
DOI:10.1007/s002400050104
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s002400050104
Volltext
Verfasserangaben:Bernhard Schummer, Michael Siegsmund, Anette Steidler, Lira Toktomambetova, Kai-Uwe Köhrmann, Peter Alken
Beschreibung
Zusammenfassung:To characterize the clinical relevance of MRP gene in the chemoresistance of prostate carcinomas we determined the multidrug resistance-associated protein (MRP) expression in 30 samples from organ-confined prostate carcinoma, 9 samples from adjacent normal tissue and 4 hormone unresponsive cancers. The measurement of MRP expression was carried out by reverse transcription polymerase chain reaction (RT-PCR) in combination with capillary electrophoresis. Incorporated fluorescence-labeled primers were disclosed by a laser-operated fluorescence detection module. MRP expression was quantified by integration of the peak area and correlated to the ubiquitously expressed β2 microglobulin. As positive control served the adriamycin-resistant HL60-ADR cell line, which overexpresses MRP. MRP expression was found in all samples. All samples showed a lower MRP/β2 ratio than HL60-ADR cells. The expression of the MRP gene was 30% higher in organ-confined tumors than in hormone-unresponsive anaplastic tumors. Normal tissue showed the same MRP mRNA level as the adriamycin-sensitive HL60 cells. A higher tumor stage correlated with an increase of MRP expression (>factor 2), whereas G3 tumors displayed a MRP expression 30% lower than in G2 tumors. The small alterations indicate that MRP expression seems not be involved in the chemoresistance of prostate carcinomas.
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Beschreibung:Online Resource
ISSN:1434-0879
DOI:10.1007/s002400050104

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