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Regulation of the extracellular signal-regulated kinase pathway in adult myocardium: differential roles of Gq/11, Gi and G12/13 proteins in signalling by α1-adrenergic, endothelin-1 and thrombin-sensitive protease-activated receptors

Using adenoviruses encoding RGS2, RGS4 and Lsc (regulator of G protein signalling (RGS) domain of p115 RhoGEF), we investigated the contributions of G(q/11), Gi and G(12/13) proteins to G protein-coupled receptor (GPCR)-mediated activation of the extracellular signal-regulated kinase (ERK) pathway i...

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Bibliographic Details
Main Authors: Snabaitis, Andrew K. (Author) , Muntendorf, Andreas (Author) , Wieland, Thomas (Author) , Avkiran, Metin (Author)
Format: Article (Journal)
Language:English
Published: 2005
In: Cellular signalling
Year: 2005, Volume: 17, Issue: 5, Pages: 655-664
ISSN:1873-3913
DOI:10.1016/j.cellsig.2004.10.008
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cellsig.2004.10.008
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Author Notes:Andrew K. Snabaitis, Andreas Muntendorf, Thomas Wieland, Metin Avkiran
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Summary:Using adenoviruses encoding RGS2, RGS4 and Lsc (regulator of G protein signalling (RGS) domain of p115 RhoGEF), we investigated the contributions of G(q/11), Gi and G(12/13) proteins to G protein-coupled receptor (GPCR)-mediated activation of the extracellular signal-regulated kinase (ERK) pathway in adult rat ventricular myocytes (ARVM). Exposure to phenylephrine, endothelin-1 (ET-1) or thrombin induced significant activation of ERK1/2 and their downstream target 90 kDa ribosomal S6 kinase (p90RSK), which was abolished by overexpression of RGS4 (inhibits signalling via G(q/11) and Gi) or RGS2 (inhibits signalling via G(q/11)). Pertussis toxin (inhibits signalling via Gi) only partially attenuated the activation of ERK1/2 and p90(RSK) by phenylephrine and ET-1, but abolished such activation by thrombin. Overexpression of Lsc (inhibits signalling via G(12/13)) did not affect the responses to phenylephrine and ET-1, but suppressed the activation of ERK1/2 and p90RSK by thrombin. We conclude that full activation of the ERK pathway in ARVM by alpha1-adrenergic, ET-1 and thrombin receptors requires the activation of distinct families of heterotrimeric G proteins.
Item Description:Available online 6 November 2004
Gesehen am 01.04.2022
Im Titel sind "q/11" in Gq/11, "i" in Gi, "12/13" in G12/13 tiefgestellt
Physical Description:Online Resource
ISSN:1873-3913
DOI:10.1016/j.cellsig.2004.10.008

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