Breast cancer cell-derived cytokines, macrophages and cell adhesion: implications for metastasis
Background: Liver metastasis is associated with a proinflammatory microenvironment and up-regulation of cell adhesion molecules expressed by endothelial cells. The aim of this study was to characterize the interrelations between breast cancer cell-secreted cytokines, macrophages and E-selectin-media...
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| Main Authors: | , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
September 30, 2011
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| In: |
Anticancer research
Year: 2011, Volume: 31, Issue: 10, Pages: 3219-3227 |
| ISSN: | 1791-7530 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://ar.iiarjournals.org/content/31/10/3219
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| Author Notes: | Christine Eichbaum, Anne-Sophie Meyer, Ni Wang, Esther Bischofs, Andrea Steinborn, Thomas Bruckner, Pnina Brodt, Christof Sohn and Michael H.R. Eichbaum |
| Summary: | Background: Liver metastasis is associated with a proinflammatory microenvironment and up-regulation of cell adhesion molecules expressed by endothelial cells. The aim of this study was to characterize the interrelations between breast cancer cell-secreted cytokines, macrophages and E-selectin-mediated cancer cell adhesion and their role in metastasis of breast cancer. Materials and Methods: Three metastatic breast cancer cell lines (1590, KM22, ZE) were studied. Cell culture supernatants were screened for cytokines and the potential for cytokines to increase tumor-necrosis factor-α (TNF-α) production by ANA-1-macrophages was analyzed. E-Selectin-mediated tumor cell adhesion of fluorescence labelled tumor cells was evaluated by measurement of fluorescence intensity with and without E-selectin-blocking strategies (monoclonal antibodies, cimetidine). Results: Tumor-specific cytokine secretion patterns were revealed. TNF-α secretion from cultured macrophages increased after incubation with tumor supernatants. Tumor cell adhesion was significantly inhibited by cimetidine and monoclonal antibodies against E-selectin (KM22 with cimetidine, p<0.05). Conclusion: Breast cancer cell-secreted cytokines stimulate macrophages to produce TNF-α, a known up-regulator of E-selectin expression, and therefore cell adherence to endothelium. Inhibition of this mechanism could be an attractive therapeutic option for the prevention of breast cnacer metastasis. |
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| Item Description: | Gesehen am 08.04.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1791-7530 |