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Improved laboratory confirmation of heparin-induced thrombocytopenia type II: time course of antibodies and combination of antigen and biologic assays

We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism unde...

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Bibliographic Details
Main Authors: Harenberg, Job (Author) , Wang, Lianchun (Author) , Hoffmann, Ursula (Author) , Huhle, Günter (Author) , Feuring, Martin (Author)
Format: Article (Journal)
Language:English
Published: 03 January 2001
In: American journal of clinical pathology
Year: 2001, Volume: 115, Issue: 3, Pages: 432-438
ISSN:1943-7722
DOI:10.1309/ET2W-5J8E-MFVU-QYAH
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1309/ET2W-5J8E-MFVU-QYAH
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Author Notes:Job Harenberg, Lianchun Wang, Ursula Hoffmann, Günter Huhle, Martin Feuring
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Summary:We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-linked immunofiltration and the carbon 14-serotonin release assays.Of the 14 patients, 11 showed positive results in both assays at day 1 after stopping heparin. The 3 patients with negative results seroconverted in one or both assays during the subsequent 7 days. Combining the positive results of the assays increased the sensitivity from 85% at day 1 to 100% at day 7. Assay results became negative in all patients within 40 days. The platelet count normalized between days 2 and 9 after withdrawal of heparin. It is assumed that the free antibody can be detected after withdrawal of heparin and after clearance of the platelet factor 4-heparin complex in patients with HIT.
Item Description:Gesehen am 26.04.2022
Physical Description:Online Resource
ISSN:1943-7722
DOI:10.1309/ET2W-5J8E-MFVU-QYAH

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