SrGAP3 interacts with lamellipodin at the cell membrane and regulates Rac-dependent cellular protrusions
SrGAP3/MEGAP is a member of the Slit-Robo GAP (srGAP) family and is implicated in repulsive axon guidance and neuronal migration through Slit-Robo-mediated signal transduction. Here we describe an inhibitory role of srGAP3 on actin dynamics, specifically on lamellipodia formation. We show that the F...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
01 December 2011
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| In: |
Journal of cell science
Year: 2011, Volume: 124, Issue: 23, Pages: 3941-3955 |
| ISSN: | 1477-9137 |
| DOI: | 10.1242/jcs.077081 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1242/jcs.077081
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| Author Notes: | Volker Endris, Lydia Haussmann, Elena Buss, Claire Bacon, Dusan Bartsch and Gudrun Rappold |
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| 520 | |a SrGAP3/MEGAP is a member of the Slit-Robo GAP (srGAP) family and is implicated in repulsive axon guidance and neuronal migration through Slit-Robo-mediated signal transduction. Here we describe an inhibitory role of srGAP3 on actin dynamics, specifically on lamellipodia formation. We show that the F-BAR domain localizes srGAP3 to the leading edge of cellular protrusions whereas the SH3 domain is important for focal adhesion targeting. We report on a novel srGAP3 interaction partner, lamellipodin, which localizes with srGAP3 at the leading edge. Live-cell analyses revealed that srGAP3 influences lamellipodin-evoked lamellipodial dynamics. Furthermore, we show that mouse embryonic fibroblasts derived from homozygous srGAP3-knockout embryos display an increased cell area and lamellipodia formation that can be blocked by shRNA-mediated knockdown of lamellipodin. | ||
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