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TaqMan-based quantification of invasive cells in the chick embryo metastasis assay

The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan® technology to quantify the invaded...

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Bibliographic Details
Main Authors: Htun van der Horst, Edward (Author) , Leupold, Jörg (Author) , Schubbert, Rainer (Author) , Ullrich, Axel (Author) , Allgayer, Heike (Author)
Format: Article (Journal)
Language:English
Published: 2004
In: BioTechniques
Year: 2004, Volume: 37, Issue: 6, Pages: 940-946
ISSN:1940-9818
DOI:10.2144/04376ST02
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.2144/04376ST02
Verlag, lizenzpflichtig, Volltext: https://www.future-science.com/doi/10.2144/04376ST02
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Author Notes:Edward Htun van der Horst, Jörg H. Leupold, Rainer Schubbert, Axel Ullrich, and Heike Allgayer
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Summary:The chick embryonic metastasis (CEM) assay is a fast in vivo method to investigate the invasive properties of tumor cells. Until now, most quantification methods were semiquantitative and time-consuming. Here we describe a rapid quantification method using TaqMan® technology to quantify the invaded tumor cells in the chorioallantoic membrane of fertilized eggs. This method is based on specific detection of human ALU sequences. Moreover, it provides high sensitivity over a wide linearity range.
Item Description:Elektronische Reproduktion der Druck-Ausgabe
Published online: 6 Jun 2018
Gesehen am 02.05.2022
Physical Description:Online Resource
ISSN:1940-9818
DOI:10.2144/04376ST02

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