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The spatial transcriptomic landscape of the healing mouse intestine following damage

The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions,...

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Main Authors: Parigi, Sara M. (Author) , Larsson, Ludvig (Author) , Das, Srustidhar (Author) , Ramirez Flores, Ricardo O. (Author) , Frede, Annika (Author) , Tripathi, Kumar P. (Author) , Diaz, Oscar E. (Author) , Selin, Katja (Author) , Morales, Rodrigo A. (Author) , Luo, Xinxin (Author) , Monasterio, Gustavo (Author) , Engblom, Camilla (Author) , Gagliani, Nicola (Author) , Sáez Rodríguez, Julio (Author) , Lundeberg, Joakim (Author) , Villablanca, Eduardo J. (Author)
Format: Article (Journal)
Language:English
Published: 11 February 2022
In: Nature Communications
Year: 2022, Volume: 13, Pages: 1-16
ISSN:2041-1723
DOI:10.1038/s41467-022-28497-0
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-022-28497-0
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-022-28497-0
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Author Notes:Sara M. Parigi, Ludvig Larsson, Srustidhar Das, Ricardo O. Ramirez Flores, Annika Frede, Kumar P. Tripathi, Oscar E. Diaz, Katja Selin, Rodrigo A. Morales, Xinxin Luo, Gustavo Monasterio, Camilla Engblom, Nicola Gagliani, Julio Saez-Rodriguez, Joakim Lundeberg & Eduardo J. Villablanca
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Summary:The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.
Item Description:Gesehen am 06.05.2022
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-022-28497-0

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