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Functional activity of HERV-K-T47D-related long terminal repeats

The human genome contains a family of endogenous retroviruses, HERV-K(HML-4), that comprises the full-length provirus HERV-K-T47D, five related elements, and hundreds of solitary long terminal repeats (LTRs). We here show that HERV-K-T47D-related LTRs are dispersed over all human chromosomes and hav...

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Bibliographic Details
Main Authors: Baust, Corinna (Author) , Seifarth, Wolfgang (Author) , Schön, Ulrike (Author) , Hehlmann, Rüdiger (Author) , Leib-Mösch, Christine (Author)
Format: Article (Journal)
Language:English
Published: 2001
In: Virology
Year: 2001, Volume: 283, Issue: 2, Pages: 262-272
ISSN:1096-0341
DOI:10.1006/viro.2001.0898
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/viro.2001.0898
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0042682201908980
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Author Notes:Corinna Baust, Wolfgang Seifarth, Ulrike Schön, Rüdiger Hehlmann, and Christine Leib-Mösch
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Summary:The human genome contains a family of endogenous retroviruses, HERV-K(HML-4), that comprises the full-length provirus HERV-K-T47D, five related elements, and hundreds of solitary long terminal repeats (LTRs). We here show that HERV-K-T47D-related LTRs are dispersed over all human chromosomes and have arisen after the divergence of Old and New World monkeys. By screening a cDNA library derived from the human mammary carcinoma cell line T47D with a HERV-K-T47D LTR probe, we isolated several clones containing LTR/cellular gene chimeras and assessed the transcriptional activity of these LTRs in transient transfection experiments. All LTRs were able to drive the expression of a reporter gene, thereby displaying distinct activities in different cell lines. We found that sequences located downstream of the LTR-U3 region modulate the level of gene expression. Based on the impact of the R region we distinguished between three different LTR types; the activity of type I LTRs was enhanced in the presence of the LTR-R region in all cell lines tested, whereas a type II LTR was downregulated. Type III LTRs are characterized by lacking or having a varying influence of the R region that was dependent on the cell line used. Finally, our results attribute to LTR-U5-gag sequences a role in determining LTR activity.
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Physical Description:Online Resource
ISSN:1096-0341
DOI:10.1006/viro.2001.0898

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