HERV-IP-T47D, a novel type C-related human endogenous retroviral sequence derived from T47D particles
A new type C retrovirus-related endogenous pol sequence (ERV-FTD) found to be occasionally copackaged in retrovirus-like particles released by the human mammary carcinoma cell line T47D was used to screen a human genomic library (Seifarth W, Skladny H, Krieg-Schneider F, Reichert A, Hehlmann R, and...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2000
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| In: |
Aids research and human retroviruses
Year: 2000, Volume: 16, Issue: 5, Pages: 471-480 |
| ISSN: | 1931-8405 |
| DOI: | 10.1089/088922200309133 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1089/088922200309133 Verlag, lizenzpflichtig, Volltext: https://www.liebertpub.com/doi/10.1089/088922200309133 
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| Author Notes: | Wolfgang Seifarth, Corinna Baust, Ulrike Schön, Anja Reichert, Rüdiger Hehlmann, and Christine Leib-Mösch |
| Summary: | A new type C retrovirus-related endogenous pol sequence (ERV-FTD) found to be occasionally copackaged in retrovirus-like particles released by the human mammary carcinoma cell line T47D was used to screen a human genomic library (Seifarth W, Skladny H, Krieg-Schneider F, Reichert A, Hehlmann R, and LeibMösch C: J Virol 1995;69:6408-6416). The DNA sequence of one full-length clone now reveals a human endogenous proviral sequence (HERV) of 4190 bp in length comprising a 5 LTR (489 bp) and regions with 37 and 74% overall amino acid homology to RTVL-Ia gag and pol genes, respectively. About 35 related elements were found to be distributed on all human chromosomes except 16, 17, and Y. Sequence comparisons with Mo-M uLV and various type C-related HERVs suggest that despite a proline primer-binding site this novel HERV element, now named HERV-IP-T47D, can be assigned to one family together with known HERV-I elements. Phylogenetic analyses of 5 proviral and 25 solitary LTR sequences confirmed the existence of two distinct but closely related subgroups of the HERV-IP superfamily in the primate genome. In contrast to most known HERV-families, the evolutionary age of HERV-IP elements dates back prior to the divergence of New and Old World monkeys. Despite their old age, members of the HERV-IP family are still transcriptionally active and were found to be highly expressed in specific human tissues such as liver and kidney. |
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| Item Description: | Elektronische Reproduktion der Druckausgabe Gesehen am 19.05.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1931-8405 |
| DOI: | 10.1089/088922200309133 |