Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis
Background/Aims: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, ß-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identi...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2002
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| In: |
Pancreatology
Year: 2002, Volume: 2, Issue: 4, Pages: 421-424 |
| ISSN: | 1424-3911 |
| DOI: | 10.1159/000065091 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000065091 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1424390302800412 
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| Author Notes: | R. Jesnowski, C. Backhaus, J. Ringel, M. Löhr |
| Summary: | Background/Aims: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, ß-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identify a housekeeping gene useful for expression analysis in pancreatic diseases. Methods: RNA isolated from various tissues and states of disease was subjected to reverse transcription and subsequently amplified by PCR using primers for GAPDH and for the ribosomal highly basic 23-kDa (rb 23kDa, RPL13A) protein. Results: As anticipated, expression of GAPDH varied markedly in the different tissues, whereas the expression of rb 23-kDa was constant in all samples investigated. Conclusion: We recommend the use of the ribosomal highly basic 23-kDa protein as a standard for normalization at least for the pancreas and the prostate. |
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| Item Description: | Elektronische Reproduktion der Druckausgabe Gesehen am 24.05.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1424-3911 |
| DOI: | 10.1159/000065091 |