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Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis

Background/Aims: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, ß-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identi...

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Bibliographic Details
Main Authors: Jesenofsky, Ralf (Author) , Backhaus, C. (Author) , Ringel, Jörg (Author) , Löhr, J.-Matthias (Author)
Format: Article (Journal)
Language:English
Published: 2002
In: Pancreatology
Year: 2002, Volume: 2, Issue: 4, Pages: 421-424
ISSN:1424-3911
DOI:10.1159/000065091
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000065091
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1424390302800412
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Author Notes:R. Jesnowski, C. Backhaus, J. Ringel, M. Löhr

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520 |a Background/Aims: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, ß-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identify a housekeeping gene useful for expression analysis in pancreatic diseases. Methods: RNA isolated from various tissues and states of disease was subjected to reverse transcription and subsequently amplified by PCR using primers for GAPDH and for the ribosomal highly basic 23-kDa (rb 23kDa, RPL13A) protein. Results: As anticipated, expression of GAPDH varied markedly in the different tissues, whereas the expression of rb 23-kDa was constant in all samples investigated. Conclusion: We recommend the use of the ribosomal highly basic 23-kDa protein as a standard for normalization at least for the pancreas and the prostate. 
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