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Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer

Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene...

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Main Authors: Alldinger, Ingo (Author) , Dittert, Dag (Author) , Peiper, Matthias (Author) , Fusco, Alberto (Author) , Chiappetta, Gennaro (Author) , Staub, Eike (Author) , Löhr, J.-Matthias (Author) , Jesenofsky, Ralf (Author) , Baretton, Gustavo Bruno (Author) , Ockert, Detlef (Author) , Saeger, Hans-Detlev (Author) , Grützmann, Robert (Author) , Pilarsky, Christian (Author)
Format: Article (Journal)
Language:English
Published: 2005
In: Pancreatology
Year: 2005, Volume: 5, Issue: 4, Pages: 370-379
ISSN:1424-3911
DOI:10.1159/000086537
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000086537
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1424390305800109
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Author Notes:Ingo Alldinger, Dag Dittert, Matthias Peiper, Alberto Fusco, Gennaro Chiappetta, Eike Staub, Matthias Löhr, Ralf Jesnowski, Gustavo Baretton, Detlef Ockert, Hans-Detlev Saeger, Robert Grützmann, Christian Pilarsky
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Summary:Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change > 3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin [β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatic tissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.
Item Description:Elektronische Reproduktion der Druckausgabe
Gesehen am 24.05.2022
Physical Description:Online Resource
ISSN:1424-3911
DOI:10.1159/000086537

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