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Solitary HERV-K LTRs possess bi-directional promoter activity and contain a negative regulatory element in the U5 region

Reporter gene analysis of HERV-K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene. Deletion analysis demonstrated the possible existence of alternative...

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Bibliographic Details
Main Authors: Domansky, Andrey N. (Author) , Kopantzev, Eugene P. (Author) , Snezhkov, Eugene V. (Author) , Lebedev, Yuri B. (Author) , Leib-Mösch, Christine (Author) , Sverdlov, Eugene D. (Author)
Format: Article (Journal)
Language:English
Published: 27 April 2000
In: FEBS letters
Year: 2000, Volume: 472, Issue: 2/3, Pages: 191-195
ISSN:1873-3468
DOI:10.1016/S0014-5793(00)01460-5
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0014-5793(00)01460-5
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/S0014-5793%2800%2901460-5
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Author Notes:Andrey N. Domansky, Eugene P. Kopantzev, Eugene V. Snezhkov, Yuri B. Lebedev, Christine Leib-Mosch, Eugene D. Sverdlov
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Summary:Reporter gene analysis of HERV-K solitary long terminal repeats (LTRs) showed that they retain detectable activity in human teratocarcinoma cells, and can direct the transcription in both orientations relative to the reporter gene. Deletion analysis demonstrated the possible existence of alternative promoters within the LTR as well as a silencer-like element in the U5 region. Our results indicate also that all-trans-retinoic acid is capable of modulating expression of the reporter gene directed by a HERV-K LTR in NT2/D1 cells.
Item Description:Gesehen am 27.05.2022
Physical Description:Online Resource
ISSN:1873-3468
DOI:10.1016/S0014-5793(00)01460-5

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