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Actomyosin-assisted pulling of lipid nanotubes from lipid vesicles and cells

Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (...

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Bibliographic Details
Main Authors: Jahnke, Kevin (Author) , Maurer, Stefan J. (Author) , Weber, Cornelia (Author) , Hernandez Bücher, Jochen Estebano (Author) , Schönit, Andreas (Author) , D'Este, Elisa (Author) , Cavalcanti-Adam, Elisabetta A. (Author) , Göpfrich, Kerstin (Author)
Format: Article (Journal)
Language:English
Published: January 28, 2022
In: Nano letters
Year: 2022, Volume: 22, Issue: 3, Pages: 1145-1150
ISSN:1530-6992
DOI:10.1021/acs.nanolett.1c04254
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acs.nanolett.1c04254
Verlag, lizenzpflichtig, Volltext: https://pubs.acs.org/doi/10.1021/acs.nanolett.1c04254
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Author Notes:Kevin Jahnke, Stefan J. Maurer, Cornelia Weber, Jochen Estebano Hernandez Bücher, Andreas Schoenit, Elisa D’Este, Elisabetta Ada Cavalcanti-Adam, and Kerstin Göpfrich
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Summary:Molecular motors are pivotal for intracellular transport as well as cell motility and have great potential to be put to use outside cells. Here, we exploit engineered motor proteins in combination with self-assembly of actin filaments to actively pull lipid nanotubes from giant unilamellar vesicles (GUVs). In particular, actin filaments are bound to the outer GUV membrane and the GUVs are seeded on a heavy meromyosin-coated substrate. Upon addition of ATP, hollow lipid nanotubes with a length of tens of micrometer are pulled from single GUVs due to the motor activity. We employ the same mechanism to pull lipid nanotubes from different types of cells. We find that the length and number of nanotubes critically depends on the cell type, whereby suspension cells form bigger networks than adherent cells. This suggests that molecular machines can be used to exert forces on living cells to probe membrane-to-cortex attachment.
Item Description:Gesehen am 13.06.2022
Published: January 28, 2022
Physical Description:Online Resource
ISSN:1530-6992
DOI:10.1021/acs.nanolett.1c04254

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