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Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA

The role of the intranuclear movement of chromatin in gene expression is not well-understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in...

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Bibliographische Detailangaben
Hauptverfasser: Chang, Lynne (VerfasserIn) , Godinez, William J. (VerfasserIn) , Kim, Il-Han (VerfasserIn) , Tektonidis, Marco (VerfasserIn) , de Lanerolle, Primal (VerfasserIn) , Eils, Roland (VerfasserIn) , Rohr, Karl (VerfasserIn) , Knipe, David M. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: May 9, 2011
In: Proceedings of the National Academy of Sciences of the United States of America
Year: 2011, Jahrgang: 108, Heft: 21, Pages: E136-E144
ISSN:1091-6490
DOI:10.1073/pnas.1103411108
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.1103411108
Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/doi/full/10.1073/pnas.1103411108
Volltext
Verfasserangaben:Lynne Chang, William J. Godinez, Il-Han Kim, Marco Tektonidis, Primal de Lanerolle, Roland Eils, Karl Rohr, and David M. Knipe
Beschreibung
Zusammenfassung:The role of the intranuclear movement of chromatin in gene expression is not well-understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in size, move, and coalesce. Quantitative analysis of RC trajectories, derived from 4D images, shows that most RCs move by directed motion. Directed movement is impaired in the presence of actin and myosin inhibitors as well as a transcription inhibitor. In addition, RCs coalesce at and reorganize nuclear speckles. Lastly, distinct effects of actin and myosin inhibitors on viral gene expression suggest that RC movement is not required for transcription, but rather, movement results in the bridging of transcriptionally active RCs with nuclear speckles to form structures that enhance export of viral late mRNAs.
Beschreibung:Gesehen am 28.06.2022
Beschreibung:Online Resource
ISSN:1091-6490
DOI:10.1073/pnas.1103411108

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