Orientation changes in surface-bound hybridized DNA undergoing preparation for ex situ spectroscopic measurements
Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and inf...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
4 August 2011
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| In: |
Chemical physics letters
Year: 2011, Volume: 513, Issue: 4, Pages: 267-270 |
| DOI: | 10.1016/j.cplett.2011.07.096 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cplett.2011.07.096 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S000926141100947X 
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| Author Notes: | Caitlin Howell, Hicham Hamoudi, Stefan Heissler, Patrick Koelsch, Michael Zharnikov |
| Summary: | Films of surface-bound homo-oligonucleotides on gold were hybridized and subjected to rinses using various volumes of water in preparation for ex situ spectroscopic analysis performed by high-resolution X-ray photoelectron spectroscopy, near-edge X-ray absorption fine structure spectroscopy, and infrared reflection-absorption spectroscopy. It was found that films rinsed with 0.5mL of water contained well-ordered DNA hybrids, while films rinsed with greater volumes (1 and 2mL) showed a decreased number of hybrids and a significant disruption of their intactness and order. These results illustrate the importance of taking into account preparation procedures when interpreting ex situ spectroscopic data of such systems. |
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| Item Description: | Gesehen am 30.06.2022 |
| Physical Description: | Online Resource |
| DOI: | 10.1016/j.cplett.2011.07.096 |