Quantum dot blueing and blinking enables fluorescence nanoscopy
We demonstrate superresolution fluorescence imaging of cells using bioconjugated CdSe/ZnS quantum dot markers. Fluorescence blueing of quantum dot cores facilitates separation of blinking markers residing closer than the diffraction barrier. The high number of successively emitted photons enables gr...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
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| In: |
Nano letters
Year: 2011, Volume: 11, Issue: 1, Pages: 245-250 |
| ISSN: | 1530-6992 |
| DOI: | 10.1021/nl103639f |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/nl103639f
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| Author Notes: | Patrick Hoyer, Thorsten Staudt, Johann Engelhardt, and Stefan W. Hell |
| Summary: | We demonstrate superresolution fluorescence imaging of cells using bioconjugated CdSe/ZnS quantum dot markers. Fluorescence blueing of quantum dot cores facilitates separation of blinking markers residing closer than the diffraction barrier. The high number of successively emitted photons enables ground state depletion microscopy followed by individual marker return with a resolving power of the size of a single dot (∼12 nm). Nanoscale imaging is feasible with a simple webcam. |
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| Item Description: | Published online3 December 2010 Gesehen am 30.06.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1530-6992 |
| DOI: | 10.1021/nl103639f |