Concurrent detection of autolysosome formation and lysosomal degradation by flow cytometry in a high-content screen for inducers of autophagy
Autophagy mediates lysosomal degradation of cytosolic components. Recent work has associated autophagic dysfunction with pathologies, including cancer and cardiovascular disease. To date, the identification of clinically-applicable drugs that modulate autophagy has been hampered by the lack of stand...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
02 June 2011
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| In: |
BMC biology
Year: 2011, Volume: 9, Pages: 1-15 |
| ISSN: | 1741-7007 |
| DOI: | 10.1186/1741-7007-9-38 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1186/1741-7007-9-38
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| Author Notes: | Phillip Hundeshagen, Anne Hamacher-Brady, Roland Eils and Nathan R. Brady |
| Summary: | Autophagy mediates lysosomal degradation of cytosolic components. Recent work has associated autophagic dysfunction with pathologies, including cancer and cardiovascular disease. To date, the identification of clinically-applicable drugs that modulate autophagy has been hampered by the lack of standardized assays capable of precisely reporting autophagic activity. |
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| Item Description: | Gesehen am 01.07.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1741-7007 |
| DOI: | 10.1186/1741-7007-9-38 |