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Characterization of a transitional preplasmablast population in the process of human B cell to plasma cell differentiation

The early steps of differentiation of human B cells into plasma cells are poorly known. We report a transitional population of CD20low/−CD38− preplasmablasts along differentiation of human memory B cells into plasma cells in vitro. Preplasmablasts lack documented B cell or plasma cell (CD20, CD38, a...

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Main Authors: Jourdan, Michel (Author) , Caraux, Anouk (Author) , Caron, Gersende (Author) , Robert, Nicolas (Author) , Fiol, Geneviève (Author) , Rème, Thierry (Author) , Bolloré, Karine (Author) , Vendrell, Jean-Pierre (Author) , Gallou, Simon Le (Author) , Mourcin, Frédéric (Author) , Vos, John De (Author) , Kassambara, Alboukadel (Author) , Duperray, Christophe (Author) , Hose, Dirk (Author) , Fest, Thierry (Author) , Tarte, Karin (Author) , Klein, Bernard (Author)
Format: Article (Journal)
Language:English
Published: October 3, 2011
In: The journal of immunology
Year: 2011, Volume: 187, Issue: 8, Pages: 3931-3941
ISSN:1550-6606
DOI:10.4049/jimmunol.1101230
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.4049/jimmunol.1101230
Verlag, lizenzpflichtig, Volltext: https://www.jimmunol.org/content/187/8/3931
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Author Notes:Michel Jourdan, Anouk Caraux, Gersende Caron, Nicolas Robert, Geneviève Fiol, Thierry Rème, Karine Bolloré, Jean-Pierre Vendrell, Simon Le Gallou, Frédéric Mourcin, John De Vos, Alboukadel Kassambara, Christophe Duperray, Dirk Hose, Thierry Fest, Karin Tarte, and Bernard Klein
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Summary:The early steps of differentiation of human B cells into plasma cells are poorly known. We report a transitional population of CD20low/−CD38− preplasmablasts along differentiation of human memory B cells into plasma cells in vitro. Preplasmablasts lack documented B cell or plasma cell (CD20, CD38, and CD138) markers, express CD30 and IL-6R, and secrete Igs at a weaker level than do plasmablasts or plasma cells. These preplasmablasts further differentiate into CD20−CD38highCD138− plasmablasts and then CD20−CD38highCD138+ plasma cells. Preplasmablasts were fully characterized in terms of whole genome transcriptome profiling and phenotype. Preplasmablasts coexpress B and plasma cell transcription factors, but at a reduced level compared with B cells, plasmablasts, or plasma cells. They express the unspliced form of XBP1 mRNA mainly, whereas plasmablasts and plasma cells express essentially the spliced form. An in vivo counterpart (CD19+CD20low/−CD38−IL-6R+ cells) of in vitro-generated preplasmablasts could be detected in human lymph nodes (0.06% of CD19+ cells) and tonsils (0.05% of CD19+ cells). An open access “B to Plasma Cell Atlas,” which makes it possible to interrogate gene expression in the process of B cell to plasma cell differentiation, is provided. Taken together, our findings show the existence of a transitional preplasmablast population using an in vitro model of plasma cell generation and of its in vivo counterpart in various lymphoid tissues.
Item Description:Gesehen am 05.07.2022
Physical Description:Online Resource
ISSN:1550-6606
DOI:10.4049/jimmunol.1101230

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