Comparison of two different radioimmunoassays to measure 17-hydroxyprogesterone during treatment monitoring of children with congenital adrenal hyperplasia due to 21-hydroxylase deficiency
Background - Accurate measurement of 17-hydroxyprogesterone (17-OHP) concentrations is essential for the correct diagnosis and treatment management of children with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (21 OHD). - Methods - We analysed 102 serum samples from 15 child...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
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| In: |
Clinica chimica acta
Year: 2011, Volume: 412, Issue: 1/2, Pages: 186-189 |
| ISSN: | 1873-3492 |
| DOI: | 10.1016/j.cca.2010.10.001 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cca.2010.10.001 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0009898110006315 
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| Author Notes: | Clemens Kamrath, Hansjosef Böhles, Christiane Maser-Gluth |
| Summary: | Background - Accurate measurement of 17-hydroxyprogesterone (17-OHP) concentrations is essential for the correct diagnosis and treatment management of children with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency (21 OHD). - Methods - We analysed 102 serum samples from 15 children with known 21 OHD twice using two different 17-OHP assays. 17-OHP concentrations were measured by an in-house radioimmunoassay (RIA) after recovery-corrected extraction and chromatographic purification and by a commercially available RIA without extraction (Immunotech). - Results - The correlation coefficient for results of pairs of 17-OHP concentrations was 0.974. The median ratio (17-OHP concentration measured with the commercial assay/17-OHP concentration measured with the in-house assay) was 0.593 with a 95% confidence interval ranging from 0.258 to 1.370. The ratio was constant throughout the average 17-OHP concentrations ranging from 0.24 to 149.2nmol/L, as well as throughout the age range from 0.3 to 16.4years. - Conclusions - Despite good overall correlation, absolute 17-OHP concentrations differed dramatically. This could lead to misclassification of patients suspected for 21 OHD on the basis of the hormonal profile and to a reduced quality during treatment monitoring of patients with 21 OHD with the risk of under- and overtreatment. |
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| Item Description: | Available online 8 October 2010 Gesehen am 07.07.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1873-3492 |
| DOI: | 10.1016/j.cca.2010.10.001 |