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Gram-positive Staphylococcus aureus LTA promotes distinct memory-like effects in murine bone marrow neutrophils

Neutrophils primarily act as first responders in acute infection and directly maintain inflammatory responses. However, a growing body of evidence suggests that neutrophils also bear the potential to mediate chronic inflammation by exhibiting memory-like features. We now asked whether bone marrow-de...

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Main Authors: Lajqi, Trim (Author) , Frommhold, David (Author) , Jungwirth, Maylis (Author) , Kranig, Simon A. (Author) , Pöschl, Johannes (Author) , Hudalla, Hannes (Author)
Format: Article (Journal)
Language:English
Published: 7 May 2022
In: Cellular immunology
Year: 2022, Volume: 376, Pages: 1-9
ISSN:1090-2163
DOI:10.1016/j.cellimm.2022.104535
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cellimm.2022.104535
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0008874922000594
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Author Notes:Trim Lajqi, David Frommhold, Maylis Braun, Simon Alexander Kranig, Johannes Pöschl, Hannes Hudalla
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Summary:Neutrophils primarily act as first responders in acute infection and directly maintain inflammatory responses. However, a growing body of evidence suggests that neutrophils also bear the potential to mediate chronic inflammation by exhibiting memory-like features. We now asked whether bone marrow-derived murine neutrophils can be primed by lipoteichoic acid (LTA) from gram-positive S. aureus. We found that low-dose (1ng/mL) LTA-priming promoted increased production of pro-inflammatory mediators (TNF-α, IL-6, ROS), whereas high-dose (10µg/mL) priming resulted in opposing reactions marked by increased IL-10 and suppressed pro-inflammatory mediators upon a second stimulus. A similar pattern of pro-inflammatory activation (trained sensitivity) and anti-inflammatory properties (tolerance) was recapitulated in cellular functional in vitro assays (transmigration and phagocytosis). Priming by LTA correlated with TLR2/MyD88-mediated regulation of NFκB-p65 through intermediate PI3Ks/MAPK. Collectively, our data suggest a previously unknown capacity of neutrophils to be differentially primed by varying doses of LTA, endorsing memory-like features in neutrophils.
Item Description:Gesehen am 13.07.2022
Physical Description:Online Resource
ISSN:1090-2163
DOI:10.1016/j.cellimm.2022.104535

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