Interleukin-17A and interleukin-22 production by conventional and non-conventional lymphocytes in three different end-stage lung diseases
Objectives The contribution of adaptive vs. innate lymphocytes to IL-17A and IL-22 secretion at the end stage of chronic lung diseases remains largely unexplored. In order to uncover tissue- and disease-specific secretion patterns, we compared production patterns of IL-17A and IL-22 in three differe...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
16 June 2022
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| In: |
Clinical & Translational Immunology
Year: 2022, Volume: 11, Issue: 6, Pages: 1-18 |
| ISSN: | 2050-0068 |
| DOI: | 10.1002/cti2.1398 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/cti2.1398 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/cti2.1398 
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| Author Notes: | Melanie Albrecht, Olga Halle, Svenja Gaedcke, Sophia T Pallenberg, Julia Camargo Neumann, Marius Witt, Johanna Roediger, Marina Schumacher, Adan Chari Jirmo, Gregor Warnecke, Danny Jonigk, Peter Braubach, David DeLuca, Gesine Hansen & Anna-Maria Dittrich |
| Summary: | Objectives The contribution of adaptive vs. innate lymphocytes to IL-17A and IL-22 secretion at the end stage of chronic lung diseases remains largely unexplored. In order to uncover tissue- and disease-specific secretion patterns, we compared production patterns of IL-17A and IL-22 in three different human end-stage lung disease entities. Methods Production of IL-17A, IL-22 and associated cytokines was assessed in supernatants of re-stimulated lymphocytes by multiplex assays and multicolour flow cytometry of conventional T cells, iNKT cells, γδ T cells and innate lymphoid cells in bronchial lymph node and lung tissue from patients with emphysema (n = 19), idiopathic pulmonary fibrosis (n = 14) and cystic fibrosis (n = 23), as well as lung donors (n = 17). Results We detected secretion of IL-17A and IL-22 by CD4+ T cells, CD8+ T cells, innate lymphoid cells, γδ T cells and iNKT cells in all end-stage lung disease entities. Our analyses revealed disease-specific contributions of individual lymphocyte subpopulations to cytokine secretion patterns. We furthermore found the high levels of microbial detection in CF samples to associate with a more pronounced IL-17A signature upon antigen-specific and unspecific re-stimulation compared to other disease entities and lung donors. Conclusion Our results show that both adaptive and innate lymphocyte populations contribute to IL-17A-dependent pathologies in different end-stage lung disease entities, where they establish an IL-17A-rich microenvironment. Microbial colonisation patterns and cytokine secretion upon microbial re-stimulation suggest that pathogens drive IL-17A secretion patterns in end-stage lung disease. |
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| Item Description: | Gesehen am 29.07.2022 |
| Physical Description: | Online Resource |
| ISSN: | 2050-0068 |
| DOI: | 10.1002/cti2.1398 |