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Monitoring neutrophil elastase and cathepsin G activity in human sputum samples

Proteases are regulators of countless physiological processes and the precise investigation of their activities remains an intriguing biomedical challenge. Among the ~600 proteases encoded by the human genome, neutrophil serine proteases (NSPs) are thoroughly investigated for their involvement in th...

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Bibliographic Details
Main Authors: Frey, Dario (Author) , Guerra, Matteo (Author) , Mall, Marcus A. (Author) , Schultz, Carsten (Author)
Format: Article (Journal) Video
Language:English
Published: May 21, 2021
In: JoVE. Video journal
Year: 2021, Issue: 171, Pages: 1-14
ISSN:1940-087X
DOI:10.3791/62193
Subjects:
Film
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.3791/62193
Verlag, lizenzpflichtig, Volltext: https://www.jove.com/de/v/62193/monitoring-neutrophil-elastase-cathepsin-g-activity-human-sputum
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Author Notes:Dario L. Frey, Matteo Guerra, Marcus A. Mall, Carsten Schultz
Description
Summary:Proteases are regulators of countless physiological processes and the precise investigation of their activities remains an intriguing biomedical challenge. Among the ~600 proteases encoded by the human genome, neutrophil serine proteases (NSPs) are thoroughly investigated for their involvement in the onset and progression of inflammatory conditions including respiratory diseases. Uniquely, secreted NSPs not only diffuse within extracellular fluids but also localize to plasma membranes. During neutrophil extracellular trap (NETs) formation, NSPs become an integral part of the secreted chromatin. Such complex behavior renders the understanding of NSPs pathophysiology a challenging task. Here, detailed protocols are shown to visualize, quantify and discriminate free and membrane-bound neutrophil elastase (NE) and cathepsin G (CG) activities in sputum samples.
Item Description:Enthält auch Textversion
Gesehen am 14.09.2022
Wissenschaftlicher Film. Deutschland. 2021
Physical Description:Online Resource
ISSN:1940-087X
DOI:10.3791/62193

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