Influence of energy and wavelength on femtosecond laser-induced nucleation of protein

The influence of energy and wavelength on femtosecond laser-induced nucleation of protein was systematically investigated with Hen Egg White Lysozyme and Glucose Isomerase at two different wavelengths, λ=780nm and 260nm. We found that the enhancement of nucleation probability at a laser wavelength o...

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Main Authors: Murai, Ryota (Author) , Yoshikawa, Hiroshi (Author) , Hasenaka, Hitoshi (Author) , Takahashi, Yoshinori (Author) , Maruyama, Mihoko (Author) , Sugiyama, Shigeru (Author) , Adachi, Hiroaki (Author) , Takano, Kazufumi (Author) , Matsumura, Hiroyoshi (Author) , Murakami, Satoshi (Author) , Inoue, Tsuyoshi (Author) , Mori, Yusuke (Author)
Format: Article (Journal)
Language:English
Published: 13 May 2011
In: Chemical physics letters
Year: 2011, Volume: 510, Issue: 1/3, Pages: 139-142
DOI:10.1016/j.cplett.2011.05.016
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.cplett.2011.05.016
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0009261411005872
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Author Notes:Ryota Murai, Hiroshi Y. Yoshikawa, Hitoshi Hasenaka, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori
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Summary:The influence of energy and wavelength on femtosecond laser-induced nucleation of protein was systematically investigated with Hen Egg White Lysozyme and Glucose Isomerase at two different wavelengths, λ=780nm and 260nm. We found that the enhancement of nucleation probability at a laser wavelength of λ=780nm was comparable with that at λ=260nm, which produces more protein dimers. The nucleation was dependent on laser pulse energy and could be induced beyond the threshold energy of cavitation bubbles. These results indicate that the photophysical processes like cavitation bubbles formation are main triggers for the femtosecond laser-induced nucleation.
Item Description:Gesehen am 15.09.2022
Physical Description:Online Resource
DOI:10.1016/j.cplett.2011.05.016