Lipid A decreases human erythrocytes deformability by increasing intracellular Ca2+: Effects of verapamil, staurosporine and the rho-kinase inhibitor Y-27632
There are several reports demonstrating an involvement of bacterial toxins in the rigidity of red blood cells (RBC). The present study investigates the influence of E. coli F-583-Rd lipid A on RBC deformability under mechanical shear stress. Verapamil (Ca2+ channel inhibitor), staurosporine (protein...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
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| In: |
Clinical hemorheology and microcirculation
Year: 2011, Volume: 49, Issue: 1/4, Pages: 315-322 |
| ISSN: | 1875-8622 |
| DOI: | 10.3233/CH-2011-1482 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.3233/CH-2011-1482 Verlag, lizenzpflichtig, Volltext: http://www.redi-bw.de/db/ebsco.php/search.ebscohost.com/login.aspx%3fdirect%3dtrue%26db%3da9h%26AN%3d70081430%26site%3dehost-live 
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| Author Notes: | P. Ruef, M. Ehrhard, D. Frommhold, L. Koch, B. Fritzsching and J. Poeschl |
| Summary: | There are several reports demonstrating an involvement of bacterial toxins in the rigidity of red blood cells (RBC). The present study investigates the influence of E. coli F-583-Rd lipid A on RBC deformability under mechanical shear stress. Verapamil (Ca2+ channel inhibitor), staurosporine (protein kinase inhibitor) and Y-27632 (rho-kinase inhibitor) were used to modify the effect of lipid A on RBC deformability. We also determined if E. coli F-583-Rd Lipid A could induce an increase of intracellular Ca2+ concentration. For the deformation measurements RBC (10 adult donors) were incubated with E. coli F-583-Rd lipid A (100μg/ml) and also co-incubated with either verapamil (10-7 mol/l), staurosporine (10-7 mol/l) or Y-27632 (10-7 mol/l). The deformation of the RBC under different shear stresses (0.6-60 Pa) was measured by a shear stress diffractometer (Rheodyne SSD). Intracellular Ca2+ was determinded by flow cytometry in RBC incubated with Lipid A and labeled with fluorescent Fluo-4/AM which binds intracellular Ca2+ with high affinity resulting in enhanced green fluorescence intensity. At increasing shear stresses Lipid A induced a significantly lower elongation. Co-incubation of the erythrocytes with verapamil or staurosporine inhibited lipid A induced decrease in elongation while Y-27632 had no effect. Verapamil, Staurosporine and Y-27632 did not influence the elongation response of the cells under control conditions. Lipid A induced a marked increase in fluorescence Fluo-4/AM indicating increased intracellular Ca2+. These results suggest that E. coli F-583-Rd lipid A is able to influence red blood cell rigidity by a rapid and significant increase of intracellular Ca2+ concentration. Verapamil and staurosporine abolished the decrease in deformability of Lipid A incubated RBC. |
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| Item Description: | Gesehen am 21.09.2022 Im Titel ist der Ausdruck "2+" hochgestellt |
| Physical Description: | Online Resource |
| ISSN: | 1875-8622 |
| DOI: | 10.3233/CH-2011-1482 |