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Marathon-induced cardiac strain as model for the evaluation of diagnostic microRNAs for acute myocardial infarction

Background: The current gold standard biomarker for myocardial infarction (MI), cardiac troponin (cTn), is recognized for its high sensitivity and organ specificity; however, it lacks diagnostic specificity. Numerous studies have introduced circulating microRNAs as potential biomarkers for MI. This...

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Main Authors: Shirvani-Samani, Omid (Author) , Scherr, Johannes (Author) , Kayvanpour, Elham (Author) , Haas, Jan (Author) , Lehmann, David Hermann (Author) , Gi, Weng-Tein (Author) , Frese, Karen S. (Author) , Nietsch, Rouven (Author) , Fehlmann, Tobias (Author) , Sandke, Steffi (Author) , Weis, Tanja (Author) , Keller, Andreas (Author) , Katus, Hugo (Author) , Halle, Martin (Author) , Frey, Norbert (Author) , Meder, Benjamin (Author) , Sedaghat-Hamedani, Farbod (Author)
Format: Article (Journal)
Language:English
Published: 2022
In: Journal of Clinical Medicine
Year: 2022, Volume: 11, Issue: 1, Pages: 1-20
ISSN:2077-0383
DOI:10.3390/jcm11010005
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/jcm11010005
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2077-0383/11/1/5
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Author Notes:Omid Shirvani Samani, Johannes Scherr, Elham Kayvanpour, Jan Haas, David H. Lehmann, Weng-Tein Gi, Karen S. Frese, Rouven Nietsch, Tobias Fehlmann, Steffi Sandke, Tanja Weis, Andreas Keller, Hugo A. Katus, Martin Halle, Norbert Frey, Benjamin Meder and Farbod Sedaghat-Hamedani
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Summary:Background: The current gold standard biomarker for myocardial infarction (MI), cardiac troponin (cTn), is recognized for its high sensitivity and organ specificity; however, it lacks diagnostic specificity. Numerous studies have introduced circulating microRNAs as potential biomarkers for MI. This study investigates the MI-specificity of these serum microRNAs by investigating myocardial stress/injury due to strenuous exercise. Methods: MicroRNA biomarkers were retrieved by comprehensive review of 109 publications on diagnostic serum microRNAs for MI. MicroRNA levels were first measured by next-generation sequencing in pooled sera from runners (n = 46) before and after conducting a full competitive marathon. Hereafter, reverse transcription quantitative real-time PCR (qPCR) of 10 selected serum microRNAs in 210 marathon runners was performed (>10,000 qPCR measurements). Results: 27 potential diagnostic microRNA for MI were retrieved by the literature review. Eight microRNAs (miR-1-3p, miR-21-5p, miR-26a-5p, miR-122-5p, miR-133a-3p, miR-142-5p, miR-191-5p, miR-486-3p) showed positive correlations with cTnT in marathon runners, whereas two miRNAs (miR-134-5p and miR-499a-5p) showed no correlations. Upregulation of miR-133a-3p (p = 0.03) and miR-142-5p (p = 0.01) went along with elevated cTnT after marathon. Conclusion: Some MI-associated microRNAs (e.g., miR-133a-3p and miR-142-5p) have similar kinetics under strenuous exercise and MI as compared to cTnT, which suggests that their diagnostic specificity could be limited. In contrast, several MI-associated microRNAs (miR-26a-5p, miR-134-5p, miR-191-5p) showed different release behavior; hence, combining cTnT with these microRNAs within a multi-marker strategy may add diagnostic accuracy in MI.
Item Description:Published: 21 December 2021
Gesehen am 27.09.2022
Physical Description:Online Resource
ISSN:2077-0383
DOI:10.3390/jcm11010005

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