Regulation of GDF-15, a distant TGF-β superfamily member, in a mouse model of cerebral ischemia
GDF-15 is a novel distant member of the TGF-β superfamily and is widely distributed in the brain and peripheral nervous system. We have previously reported that GDF-15 is a potent neurotrophic factor for lesioned dopaminergic neurons in the substantia nigra, and that GDF-15-deficient mice show progr...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
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| In: |
Cell & tissue research
Year: 2011, Volume: 343, Issue: 2, Pages: 399-409 |
| ISSN: | 1432-0878 |
| DOI: | 10.1007/s00441-010-1090-5 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00441-010-1090-5
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| Author Notes: | Katharina Schindowski, Oliver von Bohlen und Halbach, Jens Strelau, Dirk A. Ridder, Oliver Herrmann, Andreas Schober, Markus Schwaninger, Klaus Unsicker |
| Summary: | GDF-15 is a novel distant member of the TGF-β superfamily and is widely distributed in the brain and peripheral nervous system. We have previously reported that GDF-15 is a potent neurotrophic factor for lesioned dopaminergic neurons in the substantia nigra, and that GDF-15-deficient mice show progressive postnatal losses of motor and sensory neurons. We have now investigated the regulation of GDF-15 mRNA and immunoreactivity in the murine hippocampal formation and selected cortical areas following an ischemic lesion by occlusion of the middle cerebral artery (MCAO). MCAO prominently upregulates GDF-15 mRNA in the hippocampus and parietal cortex at 3 h and 24 h after lesion. GDF-15 immunoreactivity, which is hardly detectable in the unlesioned brain, is drastically upregulated in neurons identified by double-staining with NeuN. NeuN staining reveals that most, if not all, neurons in the granular layer of the dentate gyrus and pyramidal layers of the cornu ammonis become GDF-15-immunoreactive. Moderate induction of GDF-15 immunoreactivity has been observed in a small number of microglial cells identified by labeling with tomato lectin, whereas astroglial cells remain GDF-15-negative after MCAO. Comparative analysis of the size of the infarcted area after MCAO in GDF-15 wild-type and knockout mice has failed to reveal significant differences. Together, our data substantiate the notion that GDF-15 is prominently upregulated in the lesioned brain and might be involved in orchestrating post-lesional responses other than the trophic support of neurons. |
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| Item Description: | Published online: 3 December 2010 Gesehen am 11.10.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1432-0878 |
| DOI: | 10.1007/s00441-010-1090-5 |