Rational design of porous poly(ethylene glycol) films as a matrix for ssDNA immobilization and hybridization
Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on the reaction between the...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 August 2022
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| In: |
Bioengineering
Year: 2022, Volume: 9, Issue: 9, Pages: 1-16 |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering9090414 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.3390/bioengineering9090414 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2306-5354/9/9/414 
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| Author Notes: | Zhiyong Zhao, Saunak Das, Michael Zharnikov |
| Summary: | Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine- and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on the reaction between the amine groups in the films and N-hydroxy succinimide (NHS) ester groups of the NHS-ester-decorated ssDNA. Whereas the amount of reactive amine groups in the films with the standard 1:1 composition of the precursors turned out to be too low for efficient immobilization, it could be increased noticeably using an excess (2:1) concentration of the amine-terminated precursor. The respective films retained the bioinertness of the 1:1 prototype and could be successfully decorated with probe ssDNA, resulting in porous, 3D PEG-ssDNA sensing assemblies. These assemblies exhibited high selectivity with respect to the target ssDNA strands, with a hybridization efficiency of 78-89% for the matching sequences and full inertness for non-complementary strands. The respective strategy can be applied to the fabrication of DNA microarrays and DNA sensors. As a suitable transduction technique, requiring no ssDNA labeling and showing high sensitivity in the PEG-ssDNA case, electrochemical impedance spectroscopy is suggested. |
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| Item Description: | Gesehen am 18.10.2022 |
| Physical Description: | Online Resource |
| ISSN: | 2306-5354 |
| DOI: | 10.3390/bioengineering9090414 |