Molecular analysis of linezolid resistance in clinical Enterococcus faecium isolates by polymerase chain reaction and pyrosequencing
Resistance to linezolid has been associated with a G2576T mutation in the 23 S rRNA gene. Clinical isolates of linezolid-sensitive and linezolid-resistant vancomycin-resistant Enterococcus faecium of a liver transplant patient have been analysed for the G2576T mutation by polymerase chain reaction r...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
[2011]
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| In: |
European journal of clinical microbiology & infectious diseases
Year: 2011, Volume: 30, Issue: 1, Pages: 121-125 |
| ISSN: | 1435-4373 |
| DOI: | 10.1007/s10096-010-1046-4 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s10096-010-1046-4
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| Author Notes: | P. Schnitzler, K. Schulz, C. Lampson, M. Geiss, H. K. Geiss |
| Summary: | Resistance to linezolid has been associated with a G2576T mutation in the 23 S rRNA gene. Clinical isolates of linezolid-sensitive and linezolid-resistant vancomycin-resistant Enterococcus faecium of a liver transplant patient have been analysed for the G2576T mutation by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), conventional sequencing and pyrosequencing. A clear association between the number of mutated 23 S rRNA genes and the level of linezolid resistance has been demonstrated. Linezolid susceptibility re-emerged after cessation of linezolid therapy; however, the re-initiation of linezolid therapy resulted in the re-emergence of linezolid-resistant strains. Pyrosequencing rapidly detected the number of mutated alleles and is superior to conventional PCR-RFLP for the detection of heterozygous mutations. |
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| Item Description: | Published: 03 September 2010 Gesehen am 25.10.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1435-4373 |
| DOI: | 10.1007/s10096-010-1046-4 |