Establishing efficient xenograft models with intrinsic vascularisation for growing primary human low-grade sarcomas
Background: There are no xenograft models of low-grade soft tissue sarcoma. Transplant survival remains an obstacle in sarcoma xenograft models and is attributed to post-transplantation hypoxia. Models with an intrinsic tissue - engineered vascular supply may overcome this obstacle. The aim of this...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2011
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| In: |
Anticancer research
Year: 2011, Volume: 31, Issue: 12, Pages: 4061-4066 |
| ISSN: | 1791-7530 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://ar.iiarjournals.org/content/31/12/4061
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| Author Notes: | Daniel Tilkorn, Adrien Daigeler, Ingo Stricker, Angela Schaffran, Inge Schmitz, Lars Steinstraesser, Joerg Hauser, Andrej Ring, Hans-Ulrich Steinau and Sammy Al-Benna |
| Summary: | Background: There are no xenograft models of low-grade soft tissue sarcoma. Transplant survival remains an obstacle in sarcoma xenograft models and is attributed to post-transplantation hypoxia. Models with an intrinsic tissue - engineered vascular supply may overcome this obstacle. The aim of this study was to establish a novel xenograft model of primary human low grade soft tissue sarcoma. Materials and Methods: Primary low-grade liposarcoma fragments were transplanted into a silicon chamber, placed around the superficial epigastric vessels in athymic nude mice. Xenograft samples were assessed histologically (light/electron microscopy and immunohistochemistry for S100). Results: All xenotransplants of low grade primary soft tissue liposarcoma (n=4) engrafted, led to the development of solid tumours in mice. Histological and immunohistochemical staining confirmed the xenografts as being well-differentiated liposarcomas identical to the original tumor tissue. Conclusion: Successful transplantation of human low-grade liposarcoma tissue in mice was established for the first time using a model with an intrinsic vascular supply. |
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| Item Description: | Gesehen am 03.11.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1791-7530 |