Cover Image

Model based precision structural measurements on barely resolved objects

A model based method for the accurate quantification of the 3D structure of fluorescently labelled cellular objects similar in size to the optical resolution limit is presented. This method is applied to both simulated confocal images of chromatin structures and to real confocal data obtained on a F...

Full description

Saved in:
Bibliographic Details
Main Authors: Baddeley, David (Author) , Weiland, Y. (Author) , Batram, C. (Author) , Birk, U. (Author) , Cremer, Christoph (Author)
Format: Article (Journal)
Language:English
Published: 2010
In: Journal of microscopy
Year: 2010, Volume: 237, Issue: 1, Pages: 70-78
ISSN:1365-2818
DOI:10.1111/j.1365-2818.2009.03304.x
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1365-2818.2009.03304.x
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2818.2009.03304.x
Get full text
Author Notes:D. Baddeley, Y. Weiland, C. Batram, U. Birk & C. Cremer

MARC

LEADER 00000caa a2200000 c 4500
001 182386886X
003 DE-627
005 20230710162946.0
007 cr uuu---uuuuu
008 221129s2010 xx |||||o 00| ||eng c
024 7 |a 10.1111/j.1365-2818.2009.03304.x  |2 doi 
035 |a (DE-627)182386886X 
035 |a (DE-599)KXP182386886X 
035 |a (OCoLC)1389822497 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 29  |2 sdnb 
100 1 |a Baddeley, David  |e VerfasserIn  |0 (DE-588)133802116  |0 (DE-627)55638766X  |0 (DE-576)300114664  |4 aut 
245 1 0 |a Model based precision structural measurements on barely resolved objects  |c D. Baddeley, Y. Weiland, C. Batram, U. Birk & C. Cremer 
264 1 |c 2010 
300 |a 9 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Zuerst veröffentlicht: 10 Dezember 2009 
500 |a Gesehen am 29.11.2022 
520 |a A model based method for the accurate quantification of the 3D structure of fluorescently labelled cellular objects similar in size to the optical resolution limit is presented. This method is applied to both simulated confocal images of chromatin structures and to real confocal data obtained on a Fluorescence in situ Hybridization (FISH) labelled gene domain. The model assumes that the object is composed of a small number of discrete points which are convolved with the microscope point spread function to give the image. Fitting this model to image data results in a method to assess object structure which is accurate, shows a low bias, and does not require user intervention or the potentially subjective setting of a threshold. 
650 4 |a Confocal microscopy 
650 4 |a model based analysis 
650 4 |a size estimation 
700 1 |a Weiland, Y.  |e VerfasserIn  |4 aut 
700 1 |a Batram, C.  |e VerfasserIn  |4 aut 
700 1 |a Birk, U.  |e VerfasserIn  |4 aut 
700 1 |a Cremer, Christoph  |d 1944-  |e VerfasserIn  |0 (DE-588)134078799  |0 (DE-627)560220626  |0 (DE-576)300297890  |4 aut 
773 0 8 |i Enthalten in  |t Journal of microscopy  |d Oxford [u.a.] : Wiley-Blackwell, 1969  |g 237(2010), 1, Seite 70-78  |h Online-Ressource  |w (DE-627)320460959  |w (DE-600)2007259-4  |w (DE-576)091142911  |x 1365-2818  |7 nnas  |a Model based precision structural measurements on barely resolved objects 
773 1 8 |g volume:237  |g year:2010  |g number:1  |g pages:70-78  |g extent:9  |a Model based precision structural measurements on barely resolved objects 
856 4 0 |u https://doi.org/10.1111/j.1365-2818.2009.03304.x  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-2818.2009.03304.x  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20221129 
993 |a Article 
994 |a 2010 
998 |g 134078799  |a Cremer, Christoph  |m 134078799:Cremer, Christoph  |d 130000  |d 130700  |e 130000PC134078799  |e 130700PC134078799  |k 0/130000/  |k 1/130000/130700/  |p 5  |y j 
999 |a KXP-PPN182386886X  |e 4221762829 
BIB |a Y 
SER |a journal 
JSO |a {"id":{"doi":["10.1111/j.1365-2818.2009.03304.x"],"eki":["182386886X"]},"origin":[{"dateIssuedKey":"2010","dateIssuedDisp":"2010"}],"name":{"displayForm":["D. Baddeley, Y. Weiland, C. Batram, U. Birk & C. Cremer"]},"relHost":[{"title":[{"title_sort":"Journal of microscopy","title":"Journal of microscopy","subtitle":"principal publication of the Royal Microscopical Society ; official journal of the International Society for Stereology"}],"part":{"year":"2010","pages":"70-78","issue":"1","text":"237(2010), 1, Seite 70-78","volume":"237","extent":"9"},"pubHistory":["89.1969 -"],"language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"Royal Microscopical Society","role":"isb"}],"recId":"320460959","type":{"media":"Online-Ressource","bibl":"periodical"},"note":["Gesehen am 05.10.2020"],"disp":"Model based precision structural measurements on barely resolved objectsJournal of microscopy","id":{"issn":["1365-2818"],"eki":["320460959"],"doi":["10.1111/(ISSN)1365-2818"],"zdb":["2007259-4"]},"origin":[{"publisher":"Wiley-Blackwell ; Blackwell Science ; Blackwell","dateIssuedKey":"1969","dateIssuedDisp":"1969-","publisherPlace":"Oxford [u.a.] ; Oxford ; Oxford [u.a.]"}],"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"9 S."}],"title":[{"title_sort":"Model based precision structural measurements on barely resolved objects","title":"Model based precision structural measurements on barely resolved objects"}],"person":[{"given":"David","family":"Baddeley","role":"aut","roleDisplay":"VerfasserIn","display":"Baddeley, David"},{"family":"Weiland","given":"Y.","display":"Weiland, Y.","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Batram","given":"C.","roleDisplay":"VerfasserIn","display":"Batram, C.","role":"aut"},{"display":"Birk, U.","roleDisplay":"VerfasserIn","role":"aut","family":"Birk","given":"U."},{"given":"Christoph","family":"Cremer","role":"aut","display":"Cremer, Christoph","roleDisplay":"VerfasserIn"}],"recId":"182386886X","language":["eng"],"note":["Zuerst veröffentlicht: 10 Dezember 2009","Gesehen am 29.11.2022"],"type":{"bibl":"article-journal","media":"Online-Ressource"}} 
SRT |a BADDELEYDAMODELBASED2010 

Similar Items