Development and validation of an immunoluminometric assay (ilma) for fetuin-a-glycoprotein
Background: To identify dialysis patients with low fetuin-A levels, a sensitive immunoluminometric assay (ILMA) was developed. Methods: For the two-site ILMA, one monoclonal antibody was coated to polystyrene beads and one polyclonal antibody was labelled with acridinium ester. Results: The lower de...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
30 Dec 2009
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| In: |
Journal of immunoassay & immunochemistry
Year: 2009, Volume: 31, Issue: 1, Pages: 10-23 |
| ISSN: | 1532-4230 |
| DOI: | 10.1080/15321810903404830 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1080/15321810903404830
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| Author Notes: | Amelie Bäumer, Heinrich Schmidt-Gayk, Michael Aulmann, Ti-Sun Kim |
| Summary: | Background: To identify dialysis patients with low fetuin-A levels, a sensitive immunoluminometric assay (ILMA) was developed. Methods: For the two-site ILMA, one monoclonal antibody was coated to polystyrene beads and one polyclonal antibody was labelled with acridinium ester. Results: The lower detection threshold was 0.013 g/L, with the normal range 0.20-0.87 g/L (arithmetic mean 0.437 ± 0.118 g/L). Serum fetuin-A levels in the dialysis patients were significantly lower (arithmetic mean: 0.352 ± 0.099 g/L, p < 0.0001). Conclusion: This ILMA has been proved to be a reliable method for the determination of serum fetuin-A concentrations in dialysis patients. |
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| Item Description: | Gesehen am 30.11.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1532-4230 |
| DOI: | 10.1080/15321810903404830 |