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Soft X-ray holographic microscopy of chromosomes with high aspect ratio pinholes

We used digital in-line soft X-ray holography (DIXH) in the Gabor geometry to image human chromosomes. The divergent wave front was generated by diffraction of synchrotron radiation from a high aspect ratio pinhole. As under our experimental conditions the achievable resolution depends on the pinhol...

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Bibliographic Details
Main Authors: Barth, Ruth (Author) , Staier, Florian (Author) , Simpson, Todd (Author) , Mittler, Silvia (Author) , Eisebitt, Stefan (Author) , Grunze, Michael (Author) , Rosenhahn, Axel (Author)
Format: Article (Journal)
Language:English
Published: 25 March 2010
In: Journal of biotechnology
Year: 2010, Volume: 149, Issue: 4, Pages: 238-242
ISSN:1873-4863
DOI:10.1016/j.jbiotec.2010.03.017
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jbiotec.2010.03.017
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0168165610001628
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Author Notes:Ruth Barth, Florian Staier, Todd Simpson, Silvia Mittler, Stefan Eisebitt, Michael Grunze, Axel Rosenhahn
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Summary:We used digital in-line soft X-ray holography (DIXH) in the Gabor geometry to image human chromosomes. The divergent wave front was generated by diffraction of synchrotron radiation from a high aspect ratio pinhole. As under our experimental conditions the achievable resolution depends on the pinhole radius, high aspect ratio holes with diameters in the 100nm range were prepared by focused ion beam (FIB) milling. The central maximum of the obtained Airy pattern was used to image chromosomes prepared from metaphase HeLa cells at experimental resolutions of 370±40nm with soft X-rays at 260eV photon energy provided by the BESSY II synchrotron radiation facility.
Item Description:Gesehen am 06.12.2022
Physical Description:Online Resource
ISSN:1873-4863
DOI:10.1016/j.jbiotec.2010.03.017

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