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Self-segregation of myelin membrane lipids in model membranes

Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed t...

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Main Authors: Yurlova, Larisa (Author) , Kahya, Nicoletta (Author) , Aggarwal, Shweta (Author) , Kaiser, Hermann-Josef (Author) , Chiantia, Salvatore (Author) , Bakhti, Mostafa (Author) , Pewzner-Jung, Yael (Author) , Ben-David, Oshrit (Author) , Futerman, Anthony H. (Author) , Brügger, Britta (Author) , Simons, Mikael (Author)
Format: Article (Journal)
Language:English
Published: 6 December 2011
In: Biophysical journal
Year: 2011, Volume: 101, Issue: 11, Pages: 2713-2720
ISSN:1542-0086
DOI:10.1016/j.bpj.2011.10.026
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.bpj.2011.10.026
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006349511012471
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Author Notes:Larisa Yurlova, Nicoletta Kahya, Shweta Aggarwal, Hermann-Josef Kaiser, Salvatore Chiantia, Mostafa Bakhti, Yael Pewzner-Jung, Oshrit Ben-David, Anthony H. Futerman, Britta Brügger, and Mikael Simons
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Summary:Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical properties of myelin membranes prepared from wild-type mice and from mouse mutants that are unable to form stable myelin. Using C-Laurdan and fluorescence correlation spectroscopy, we find that lipids are tightly organized and highly ordered in myelin isolated from wild-type mice, but not from shiverer and ceramide synthase 2 null mice. Furthermore, only myelin lipids from wild-type mice laterally segregate into physically distinct lipid phases in giant unilamellar vesicles in a process that requires very long chain glycosphingolipids. Taken together, our findings suggest that oligodendrocytes exploit the potential of lipids to self-segregate to generate a highly ordered membrane for electrical insulation of axons.
Item Description:Gesehen am 19.12.2022
Physical Description:Online Resource
ISSN:1542-0086
DOI:10.1016/j.bpj.2011.10.026

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