Role of magnesium ions in DNA recognition by the EcoRV restriction endonuclease
The restriction endonuclease EcoRV binds two magnesium ions. One of these ions, , binds to the phosphate group where the cleavage occurs and is required for catalysis, but the role of the other ion, is debated. Here, multiple independent molecular dynamics simulations suggest that is crucial for ach...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
5 August 2011
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| In: |
FEBS letters
Year: 2011, Volume: 585, Issue: 17, Pages: 2739-2743 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2011.07.036 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.febslet.2011.07.036 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2011.07.036 
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| Author Notes: | Mai Zahran, Tomasz Berezniak, Petra Imhof, Jeremy C. Smith |
| Summary: | The restriction endonuclease EcoRV binds two magnesium ions. One of these ions, , binds to the phosphate group where the cleavage occurs and is required for catalysis, but the role of the other ion, is debated. Here, multiple independent molecular dynamics simulations suggest that is crucial for achieving a tightly bound protein-DNA complex and stabilizing a conformation that allows cleavage. In the absence of in all simulations the protein-DNA hydrogen bond network is significantly disrupted and the sharp kink at the central base pair step of the DNA, which is observed in the two-metal complex, is not present. Also, the active site residues rearrange in such a way that the formation of a nucleophile, required for DNA hydrolysis, is unlikely. |
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| Item Description: | Gesehen am 19.12.2022 |
| Physical Description: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/j.febslet.2011.07.036 |