Balancing the length of the distal tip by septins is key for stability and signalling function of primary cilia
Abstract Primary cilia are antenna-like organelles required for signalling transduction. How cilia structure is mechanistically maintained at steady-state to promote signalling is largely unknown. Here, we define that mammalian primary cilia axonemes are formed by proximal segment (PS) and distal se...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2022
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| In: |
The EMBO journal
Year: 2022, Volume: 41, Pages: 1-19 |
| ISSN: | 1460-2075 |
| DOI: | 10.15252/embj.2021108843 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.15252/embj.2021108843 Verlag, kostenfrei, Volltext: https://www.embopress.org/doi/full/10.15252/embj.2021108843 
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| Author Notes: | Taishi Kanamaru, Annett Neuner, Bahtiyar Kurtulmus & Gislene Pereira |
| Summary: | Abstract Primary cilia are antenna-like organelles required for signalling transduction. How cilia structure is mechanistically maintained at steady-state to promote signalling is largely unknown. Here, we define that mammalian primary cilia axonemes are formed by proximal segment (PS) and distal segment (DS) delineated by tubulin polyglutamylation-rich and -poor regions, respectively. The analysis of proximal/distal segmentation indicated that perturbations leading to cilia over-elongation influenced PS or DS length with a different impact on cilia behaviour. We identified septins as novel repressors of DS growth. We show that septins control the localisation of MKS3 and CEP290 required for a functional transition zone (TZ), and the cilia tip accumulation of the microtubule-capping kinesin KIF7, a cilia-growth inhibitor. Live-cell imaging and analysis of sonic-hedgehog (SHH) signalling activation established that DS over-extension increased cilia ectocytosis events and decreased SHH activation. Our data underlines the importance of understanding cilia segmentation for length control and cilia-dependent signalling. |
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| Item Description: | Published online 22 November 2021 Gesehen am 04.01.2023 |
| Physical Description: | Online Resource |
| ISSN: | 1460-2075 |
| DOI: | 10.15252/embj.2021108843 |