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Validated UPLC-MS/MS method for the analysis of vitamin B6 pyridoxal 5́-phosphate, pyridoxal, pyridoxine, pyridoxamine, and pyridoxic acid in human cerebrospinal fluid

Vitamin B6 and its metabolites play a crucial role in the development and interaction of brain metabolism. Following diagnostic improvements additional inherited disorders in vitamin B6 metabolism have been identified, most of them leading to a severe epileptic disorder accompanied by progressive ne...

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Main Authors: Rossmann, Julia (Author) , Christ, Stine (Author) , Richter, Sylvia (Author) , Garbade, Sven (Author) , Hoffmann, Georg F. (Author) , Opladen, Thomas (Author) , Okun, Jürgen G. (Author)
Format: Article (Journal)
Language:English
Published: 14 October 2022
In: Journal of chromatography
Year: 2022, Volume: 1212, Pages: 1-9
ISSN:1873-376X
DOI:10.1016/j.jchromb.2022.123503
Online Access:Verlag, Volltext: https://doi.org/10.1016/j.jchromb.2022.123503
Verlag, Volltext: https://www.sciencedirect.com/science/article/pii/S1570023222004081
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Author Notes:Julia Rossmann, Stine Christ, Sylvia Richter, Sven Friedrich Garbade, Georg Friedrich Hoffmann, Thomas Opladen, Jürgen Günther Okun
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Summary:Vitamin B6 and its metabolites play a crucial role in the development and interaction of brain metabolism. Following diagnostic improvements additional inherited disorders in vitamin B6 metabolism have been identified, most of them leading to a severe epileptic disorder accompanied by progressive neurological deficits including intellectual disability and microcephaly. Since early treatment can improve the outcome, fast and reliable detection of metabolic biomarkers is important. Therefore, the analysis of vitamin B6 metabolites has become increasingly important, but is, however, still challenging and limited to a few specialized laboratories. Until today, vitamin B6 metabolites are measured by liquid chromatography tandem mass spectrometry (LC-MS/MS) using trichloroacetic acid for protein precipitation. In this work, we present the development and validation of a new, accurate and reliable method for analysis and quantification of the vitamin B6 vitamers pyridoxal 5́-phosphate (PLP), pyridoxal (PL), pyridoxine (PN), pyridoxamine (PM) and pyridoxic acid (PA) in human CSF samples using acetonitrile for protein precipitation.
Item Description:Gesehen am 18.01.2023
Physical Description:Online Resource
ISSN:1873-376X
DOI:10.1016/j.jchromb.2022.123503

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