Hypoxia induces late preconditioning in the rat heart in vivo
Although hypoxic late preconditioning (LPC) limits ischemia-reperfusion injury in vitro, its cardioprotective effect is not established in vivo.In part 1, rats were exposed to 4 h of hypoxia (16%, 12%, 8% oxygen) before 24 h of reoxygenation. In part 2, normoxic rats received early preconditioning w...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
December 2010
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| In: |
Anesthesiology
Year: 2010, Volume: 113, Issue: 6, Pages: 1351-1360 |
| ISSN: | 1528-1175 |
| DOI: | 10.1097/ALN.0b013e3181fce7ea |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1097/ALN.0b013e3181fce7ea
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| Author Notes: | Marc M. Berger, M.D., Ragnar Huhn, M.D., Ph.D., Gezina T. Oei, B.Sc., M.Sc., André Heinen, M.D., Ph.D., Andreas Winzer, M.D., Inge Bauer, Ph.D., Benedikt Preckel, M.D., M.A., D.E.A.A., Nina C. Weber, Ph.D., Wolfgang Schlack, M.D., D.E.A.A., Markus W. Hollmann, M.D., Ph.D., D.E.A.A. |
| Summary: | Although hypoxic late preconditioning (LPC) limits ischemia-reperfusion injury in vitro, its cardioprotective effect is not established in vivo.In part 1, rats were exposed to 4 h of hypoxia (16%, 12%, 8% oxygen) before 24 h of reoxygenation. In part 2, normoxic rats received early preconditioning with sevoflurane (1 minimum alveolar concentration [MAC] for 3 × 5 min), continuous administration of 1 MAC sevoflurane, or 11 mg · kg · h propofol. Thereafter, all rats underwent 25 min of regional myocardial ischemia and 120 min of reperfusion. After reperfusion, hearts were excised for infarct staining. The expression of protein kinase C (PKC)α and PKCε was assessed by Western blot analysis and the expression of heme oxygenase-1 and vascular endothelial growth factor by reverse transcriptase polymerase chain reaction.In normoxic control rats, infarct size was 62 ± 6% of the area at risk. Hypoxic LPC reduced infarct size (LPC16: 36 ± 11%, LPC12: 38 ± 10%, LPC8: 39 ± 11%; each P < 0.001) to approximately the same magnitude as sevoflurane-preconditioning (40 ± 8%; P < 0.001). Combined LPC16 and sevoflurane preconditioning was not superior to either substance alone. Continuous sevoflurane or propofol was not protective. The PKC inhibitor calphostin C abolished the cardioprotective effects of LPC16. PKCε, but not PKCα, expression was increased 6 and 28 h after hypoxic LPC. Heme oxygenase-1 and vascular endothelial growth factor were transiently up-regulated after 6 h.Hypoxic LPC at 8%, 12%, and 16% oxygen reduces infarct size in the rat heart in vivo. This effect is as powerful as sevoflurane-preconditioning. PKCε is a key player in mediating hypoxic LPC. |
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| Item Description: | Gesehen am 18.01.2023 |
| Physical Description: | Online Resource |
| ISSN: | 1528-1175 |
| DOI: | 10.1097/ALN.0b013e3181fce7ea |