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Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release

Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discovered CMS1 as a null suppres...

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Main Authors: Lau, Benjamin (Author) , Beine-Golovchuk, Olga (Author) , Kornprobst, Markus (Author) , Cheng, Jingdong (Author) , Kressler, Dieter (Author) , Jády, Beáta (Author) , Kiss, Tamás (Author) , Beckmann, Roland (Author) , Hurt, Ed (Author)
Format: Article (Journal)
Language:English
Published: 22 November 2022
In: Cell reports
Year: 2022, Volume: 41, Issue: 8, Pages: 1-22
ISSN:2211-1247
DOI:10.1016/j.celrep.2022.111684
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2022.111684
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2211124722015583
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Author Notes:Benjamin Lau, Olga Beine-Golovchuk, Markus Kornprobst, Jingdong Cheng, Dieter Kressler, Beáta Jády, Tamás Kiss, Roland Beckmann, Ed Hurt
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Summary:Ribosome synthesis begins in the nucleolus with 90S pre-ribosome construction, but little is known about how the many different snoRNAs that modify the pre-rRNA are timely guided to their target sites. Here, we report a role for Cms1 in such a process. Initially, we discovered CMS1 as a null suppressor of a nop14 mutant impaired in Rrp12-Enp1 factor recruitment to the 90S. Further investigations detected Cms1 at the 18S rRNA 3′ major domain of an early 90S that carried H/ACA snR83, which is known to guide pseudouridylation at two target sites within the same subdomain. Cms1 co-precipitates with many 90S factors, but Rrp12-Enp1 encircling the 3′ major domain in the mature 90S is decreased. We suggest that Cms1 associates with the 3′ major domain during early 90S biogenesis to restrict premature Rrp12-Enp1 binding but allows snR83 to timely perform its modification role before the next 90S assembly steps coupled with Cms1 release take place.
Item Description:Gesehen am 23.01.2023
Physical Description:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2022.111684

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