Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular...

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Hauptverfasser: Fung, Herman K. H. (Verfasst von) , Hayashi, Yuki (Verfasst von) , Salo, Veijo T. (Verfasst von) , Babenko, Anastasiia (Verfasst von) , Zagoriy, Ievgeniia (Verfasst von) , Brunner, Andreas (Verfasst von) , Ellenberg, Jan (Verfasst von) , Müller, Christoph W. (Verfasst von) , Cuylen-Haering, Sara (Verfasst von) , Mahamid, Julia (Verfasst von)
Dokumenttyp: Article (Journal) Kapitel/Artikel
Sprache:Englisch
Veröffentlicht: December 10, 2022
In: bioRxiv beta
Year: 2022, Pages: 1-29
DOI:10.1101/2022.12.10.519870
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1101/2022.12.10.519870
Verlag, lizenzpflichtig, Volltext: https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1
Volltext
Verfasserangaben:Herman KH Fung, Yuki Hayashi, Veijo T. Salo, Anastasiia Babenko, Ievgeniia Zagoriy, Andreas Brunner, Jan Ellenberg, Christoph W. Müller, Sara Cuylen-Haering, Julia Mahamid
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Zusammenfassung:Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells.
Beschreibung:Gesehen am 02.02.2023
Beschreibung:Online Resource
DOI:10.1101/2022.12.10.519870