Cover Image

Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM

Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular...

Full description

Saved in:
Bibliographic Details
Main Authors: Fung, Herman K. H. (Author) , Hayashi, Yuki (Author) , Salo, Veijo T. (Author) , Babenko, Anastasiia (Author) , Zagoriy, Ievgeniia (Author) , Brunner, Andreas (Author) , Ellenberg, Jan (Author) , Müller, Christoph W. (Author) , Cuylen-Haering, Sara (Author) , Mahamid, Julia (Author)
Format: Article (Journal) Chapter/Article
Language:English
Published: December 10, 2022
In: bioRxiv beta
Year: 2022, Pages: 1-29
DOI:10.1101/2022.12.10.519870
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1101/2022.12.10.519870
Verlag, lizenzpflichtig, Volltext: https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1
Get full text
Author Notes:Herman KH Fung, Yuki Hayashi, Veijo T. Salo, Anastasiia Babenko, Ievgeniia Zagoriy, Andreas Brunner, Jan Ellenberg, Christoph W. Müller, Sara Cuylen-Haering, Julia Mahamid
Description
Summary:Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells.
Item Description:Gesehen am 02.02.2023
Physical Description:Online Resource
DOI:10.1101/2022.12.10.519870

Similar Items