Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
December 10, 2022
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| In: |
bioRxiv beta
Year: 2022, Pages: 1-29 |
| DOI: | 10.1101/2022.12.10.519870 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1101/2022.12.10.519870 Verlag, lizenzpflichtig, Volltext: https://www.biorxiv.org/content/10.1101/2022.12.10.519870v1 
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| Verfasserangaben: | Herman KH Fung, Yuki Hayashi, Veijo T. Salo, Anastasiia Babenko, Ievgeniia Zagoriy, Andreas Brunner, Jan Ellenberg, Christoph W. Müller, Sara Cuylen-Haering, Julia Mahamid |
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| 520 | |a Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells. | ||
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