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DNA methylation pattern changes upon long-term culture and aging of human mesenchymal stromal cells

Within 2-3 months of in vitro culture-expansion, mesenchymal stromal cells (MSC) undergo replicative senescence characterized by cell enlargement, loss of differentiation potential and ultimate growth arrest. In this study, we have analyzed DNA methylation changes upon long-term culture of MSC by us...

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Bibliographic Details
Main Authors: Bork, Simone (Author) , Pfister, Stefan (Author) , Witt, Hendrik (Author) , Horn, Patrick (Author) , Korn, Bernhard (Author) , Ho, Anthony Dick (Author) , Wagner, Wolfgang (Author)
Format: Article (Journal)
Language:English
Published: 11 January 2010
In: Aging cell
Year: 2010, Volume: 9, Issue: 1, Pages: 54-63
ISSN:1474-9726
DOI:10.1111/j.1474-9726.2009.00535.x
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/j.1474-9726.2009.00535.x
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1474-9726.2009.00535.x
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Author Notes:Simone Bork, Stefan Pfister, Hendrik Witt, Patrick Horn, Bernhard Korn, Anthony D. Ho and Wolfgang Wagner
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Summary:Within 2-3 months of in vitro culture-expansion, mesenchymal stromal cells (MSC) undergo replicative senescence characterized by cell enlargement, loss of differentiation potential and ultimate growth arrest. In this study, we have analyzed DNA methylation changes upon long-term culture of MSC by using the HumanMethylation27 BeadChip microarray assessing 27 578 unique CpG sites. Furthermore, we have compared MSC from young and elderly donors. Overall, methylation patterns were maintained throughout both long-term culture and aging but highly significant differences were observed at specific CpG sites. Many of these differences were observed in homeobox genes and genes involved in cell differentiation. Methylation changes were verified by pyrosequencing after bisulfite conversion and compared to gene expression data. Notably, methylation changes in MSC were overlapping in long-term culture and aging in vivo. This supports the notion that replicative senescence and aging represent developmental processes that are regulated by specific epigenetic modifications.
Item Description:Gesehen am 09.02.2023
Physical Description:Online Resource
ISSN:1474-9726
DOI:10.1111/j.1474-9726.2009.00535.x

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