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Enhancement of radiation response in osteosarcoma and rhabomyosarcoma cell lines by histone deacetylase inhibition

Purpose - Histone deacetylase inhibitors (HDACIs) can enhance the sensitivity of cells to photon radiation treatment (XRT) by altering numerous molecular pathways. We investigated the effect of pan-HDACIs such as suberoylanilide hydroxamic acid (SAHA) on radiation response in two osteosarcoma (OS) a...

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Main Authors: Blattmann, Claudia (Author) , Oertel, Susanne (Author) , Ehemann, Volker (Author) , Thiemann, Markus (Author) , Huber, Peter E. (Author) , Bischof, Marc (Author) , Witt, Olaf (Author) , Deubzer, Hedwig (Author) , Kulozik, Andreas (Author) , Debus, Jürgen (Author) , Weber, Klaus-Josef (Author)
Format: Article (Journal)
Language:English
Published: March 19, 2010
In: International journal of radiation oncology, biology, physics
Year: 2010, Volume: 78, Issue: 1, Pages: 237-245
ISSN:1879-355X
DOI:10.1016/j.ijrobp.2010.03.010
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ijrobp.2010.03.010
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0360301610004141
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Author Notes:CLAUDIA BLATTMANN, M.D., SUSANNE OERTEL, M.D., VOLKER EHEMANN, PH.D., MARKUS THIEMANN, PH.D., PETER E. HUBER, M.D., MARC BISCHOF, M.D., OLAF WITT, M.D., HEDWIG E. DEUBZER, M.D., ANDREAS E. KULOZIK, M.D., PH.D., JÜRGEN DEBUS, M.D., PH.D., AND KLAUS-J. WEBER, PH.D.
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Summary:Purpose - Histone deacetylase inhibitors (HDACIs) can enhance the sensitivity of cells to photon radiation treatment (XRT) by altering numerous molecular pathways. We investigated the effect of pan-HDACIs such as suberoylanilide hydroxamic acid (SAHA) on radiation response in two osteosarcoma (OS) and two rhabdomyosarcoma (RMS) cell lines. - Methods and Materials - Clonogenic survival, cell cycle analysis, and apoptosis were examined in OS (KHOS-24OS, SAOS2) and RMS (A-204, RD) cell lines treated with HDACI and HDACI plus XRT, respectively. Protein expression was investigated via immunoblot analysis, and cell cycle analysis and measurement of apoptosis were performed using flow cytometry. - Results - SAHA induced an inhibition of cell proliferation and clonogenic survival in OS and RMS cell lines and led to a significant radiosensitization of all tumor cell lines. Other HDACI such as M344 and valproate showed similar effects as investigated in one OS cell line. Furthermore, SAHA significantly increased radiation-induced apoptosis in the OS cell lines, whereas in the RMS cell lines radiation-induced apoptosis was insignificant with and without SAHA. In all investigated sarcoma cell lines, SAHA attenuated radiation-induced DNA repair protein expression (Rad51, Ku80). - Conclusion - Our results show that HDACIs enhance radiation action in OS and RMS cell lines. Inhibition of DNA repair, as well as increased apoptosis induction after exposure to HDACIs, can be mechanisms of radiosensitization by HDACIs.
Item Description:Gesehen am 14.02.2023
Physical Description:Online Resource
ISSN:1879-355X
DOI:10.1016/j.ijrobp.2010.03.010

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