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Correlative Raman-Electron-Light (CREL) microscopy analysis of lipid droplets in melanoma cancer stem cells

Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging tech...

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Main Authors: Pagliari, Francesca (Author) , Sogne, Elisa (Author) , Panella, Davide (Author) , Perozziello, Gerardo (Author) , Liberale, Carlo (Author) , Das, Gobind (Author) , Turdo, Alice (Author) , Di Franco, Simone (Author) , Seco, Joao (Author) , Falqui, Andrea (Author) , Gratteri, Santo (Author) , Pujia, Arturo (Author) , Di Fabrizio, Enzo (Author) , Candeloro, Patrizio (Author) , Tirinato, Luca (Author)
Format: Article (Journal)
Language:English
Published: 1 December 2022
In: Biosensors
Year: 2022, Volume: 12, Issue: 12, Pages: 1-12
ISSN:2079-6374
DOI:10.3390/bios12121102
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/bios12121102
Verlag, kostenfrei, Volltext: https://www.mdpi.com/2079-6374/12/12/1102
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Author Notes:Francesca Pagliari, Elisa Sogne, Davide Panella, Gerardo Perozziello, Carlo Liberale, Gobind Das, Alice Turdo, Simone Di Franco, Joao Seco, Andrea Falqui, Santo Gratteri, Arturo Pujia, Enzo Di Fabrizio, Patrizio Candeloro and Luca Tirinato
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Summary:Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light-electron microscopy and by correlative Raman imaging-scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).
Item Description:Gesehen am 09.11.2023
Physical Description:Online Resource
ISSN:2079-6374
DOI:10.3390/bios12121102

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