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A large-scale RNAi screen identifies Deaf1 as a regulator of innate immune responses in Drosophila

Innate immune signalling pathways are evolutionarily conserved between invertebrates and vertebrates. The analysis of NF-ĸB signalling in Drosophila has contributed important insights into how organisms respond to infection. Nevertheless, significant gaps remain in our understanding of how the activ...

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Main Authors: Kuttenkeuler, David (Author) , Pelte, Nadège (Author) , Ragab, Anan (Author) , Gesellchen, Viola (Author) , Schneider, Lena (Author) , Blass, Claudia (Author) , Axelsson, Elin (Author) , Huber, Wolfgang (Author) , Boutros, Michael (Author)
Format: Article (Journal)
Language:English
Published: February 2010
In: Journal of innate immunity
Year: 2010, Volume: 2, Issue: 2, Pages: 181-194
ISSN:1662-8128
DOI:10.1159/000248649
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000248649
Verlag, lizenzpflichtig, Volltext: https://www.karger.com/Article/FullText/248649
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Author Notes:David Kuttenkeuler, Nadège Pelte, Anan Ragab, Viola Gesellchen, Lena Schneider, Claudia Blass, Elin Axelsson, Wolfgang Huber, Michael Boutros
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Summary:Innate immune signalling pathways are evolutionarily conserved between invertebrates and vertebrates. The analysis of NF-ĸB signalling in Drosophila has contributed important insights into how organisms respond to infection. Nevertheless, significant gaps remain in our understanding of how the activation of intracellular signalling elicits specific transcriptional programs. Here we report a genome-wide RNA interference survey for transcription factors that are required for Toll-dependent immune responses. In addition to the NF-ĸB homologs Dif, Dorsal and factors of the general transcription machinery, we identified Deformed Epidermal Autoregulatory Factor 1 (Deaf1) to be required for the expression of the Toll target gene Drosomycin in cultured cells and in Drosophila in vivo. We show that Deaf1 is required for the survival of flies after fungal, but not E. coli, infection. We determine that Deaf1 acts downstream of the NF-ĸB factors Dorsal and Dif. These results indicate that Deaf1 is an important contributor to innate immune responses in vivo.
Item Description:Online veröffentlicht: Oktober 13, 2009
Gesehen am 09.03.2023
Physical Description:Online Resource
ISSN:1662-8128
DOI:10.1159/000248649

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