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SCFFbxw5 targets kinesin-13 proteins to facilitate ciliogenesis

Microtubule depolymerases of the kinesin-13 family play important roles in various cellular processes and are frequently overexpressed in different cancer types. Despite the importance of their correct abundance, remarkably little is known about how their levels are regulated in cells. Using compreh...

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Main Authors: Schweiggert, Jörg (Author) , Habeck, Gregor (Author) , Hess, Sandra (Author) , Mikus, Felix (Author) , Beloshistov, Roman (Author) , Meese, Klaus (Author) , Hata, Shoji (Author) , Knobeloch, Klaus-Peter (Author) , Melchior, Frauke (Author)
Format: Article (Journal)
Language:English
Published: 9 August 2021
In: The EMBO journal
Year: 2021, Volume: 40, Issue: 18, Pages: 1-20
ISSN:1460-2075
DOI:10.15252/embj.2021107735
Online Access:Resolving-System, kostenfrei, Volltext: https://doi.org/10.15252/embj.2021107735
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Author Notes:Jörg Schweiggert, Gregor Habeck, Sandra Hess, Felix Mikus, Roman Beloshistov, Klaus Meese, Shoji Hata, Klaus-Peter Knobeloch & Frauke Melchior
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Summary:Microtubule depolymerases of the kinesin-13 family play important roles in various cellular processes and are frequently overexpressed in different cancer types. Despite the importance of their correct abundance, remarkably little is known about how their levels are regulated in cells. Using comprehensive screening on protein microarrays, we identified 161 candidate substrates of the multi-subunit ubiquitin E3 ligase SCFFbxw5 , including the kinesin-13 member Kif2c/MCAK. In vitro reconstitution assays demonstrate that MCAK and its closely related orthologs Kif2a and Kif2b become efficiently polyubiquitylated by neddylated SCFFbxw5 and Cdc34, without requiring preceding modifications. In cells, SCFFbxw5 targets MCAK for proteasomal degradation predominantly during G2 . While this seems largely dispensable for mitotic progression, loss of Fbxw5 leads to increased MCAK levels at basal bodies and impairs ciliogenesis in the following G1 /G0 , which can be rescued by concomitant knockdown of MCAK, Kif2a or Kif2b. We thus propose a novel regulatory event of ciliogenesis that begins already within the G2 phase of the preceding cell cycle.
Item Description:Im Titel ist der Ausdruck Fbxw5 hochgestellt
Gesehen am 13.03.2023
Physical Description:Online Resource
ISSN:1460-2075
DOI:10.15252/embj.2021107735

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