Cover Image

Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2

FGF2 is a cell survival factor involved in tumor-induced angiogenesis that is secreted through an unconventional secretory pathway based upon direct protein translocation across the plasma membrane. Here, we demonstrate that both PI(4,5)P2-dependent FGF2 recruitment at the inner plasma membrane leaf...

Full description

Saved in:
Bibliographic Details
Main Authors: Lolicato, Fabio (Author) , Saleppico, Roberto (Author) , Griffo, Alessandra (Author) , Meyer, Annalena (Author) , Scollo, Federica (Author) , Pokrandt, Bianca (Author) , Müller, Hans-Michael (Author) , Ewers, Helge (Author) , Hähl, Hendrik (Author) , Fleury, Jean-Baptiste (Author) , Seemann, Ralf (Author) , Hof, Martin (Author) , Brügger, Britta (Author) , Jacobs, Karin (Author) , Vattulainen, Ilpo (Author) , Nickel, Walter (Author)
Format: Article (Journal)
Language:English
Published: September 29 2022
In: The journal of cell biology
Year: 2022, Volume: 221, Issue: 11, Pages: 1-29
ISSN:1540-8140
DOI:10.1083/jcb.202106123
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1083/jcb.202106123
Get full text
Author Notes:Fabio Lolicato, Roberto Saleppico, Alessandra Griffo, Annalena Meyer, Federica Scollo, Bianca Pokrandt, Hans-Michael Müller, Helge Ewers, Hendrik Hähl, Jean-Baptiste Fleury, Ralf Seemann, Martin Hof, Britta Brügger, Karin Jacobs, Ilpo Vattulainen, and Walter Nickel
Description
Summary:FGF2 is a cell survival factor involved in tumor-induced angiogenesis that is secreted through an unconventional secretory pathway based upon direct protein translocation across the plasma membrane. Here, we demonstrate that both PI(4,5)P2-dependent FGF2 recruitment at the inner plasma membrane leaflet and FGF2 membrane translocation into the extracellular space are positively modulated by cholesterol in living cells. We further revealed cholesterol to enhance FGF2 binding to PI(4,5)P2-containing lipid bilayers. Based on extensive atomistic molecular dynamics (MD) simulations and membrane tension experiments, we proposed cholesterol to modulate FGF2 binding to PI(4,5)P2 by (i) increasing head group visibility of PI(4,5)P2 on the membrane surface, (ii) increasing avidity by cholesterol-induced clustering of PI(4,5)P2 molecules triggering FGF2 oligomerization, and (iii) increasing membrane tension facilitating the formation of lipidic membrane pores. Our findings have general implications for phosphoinositide-dependent protein recruitment to membranes and explain the highly selective targeting of FGF2 toward the plasma membrane, the subcellular site of FGF2 membrane translocation during unconventional secretion of FGF2.
Item Description:Im Titel ist die Zahl 2 im Ausdruck "PI(4,5)P2" tiefgestellt
Gesehen am 21.03.2023
Physical Description:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.202106123

Similar Items