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Deuteration distribution estimation with improved sequence coverage for HX/MS experiments

Time-resolved hydrogen exchange (HX) followed by mass spectrometry (MS) is a key technology for studying protein structure, dynamics and interactions. HX experiments deliver a time-dependent distribution of deuteration levels of peptide sequences of the protein of interest. The robust and complete e...

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Main Authors: Lou, Xinghua (Author) , Kirchner, Marc (Author) , Renard, Bernhard Y. (Author) , Köthe, Ullrich (Author) , Boppel, Sebastian (Author) , Graf, Christian (Author) , Lee, Chung-Tien (Author) , Steen, Judith A. J. (Author) , Steen, Hanno (Author) , Mayer, Matthias P. (Author) , Hamprecht, Fred (Author)
Format: Article (Journal)
Language:English
Published: May 3, 2010
In: Bioinformatics
Year: 2010, Volume: 26, Issue: 12, Pages: 1535-1541
ISSN:1367-4811
DOI:10.1093/bioinformatics/btq165
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1093/bioinformatics/btq165
Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/bioinformatics/article/26/12/1535/281316?login=true
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Author Notes:Xinghua Lou, Marc Kirchner, Bernhard Y. Renard, Ullrich Köthe, Sebastian Boppel, Christian Graf, Chung-Tien Lee, Judith A.J. Steen, Hanno Steen, Matthias P. Mayer and Fred A. Hamprecht
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Summary:Time-resolved hydrogen exchange (HX) followed by mass spectrometry (MS) is a key technology for studying protein structure, dynamics and interactions. HX experiments deliver a time-dependent distribution of deuteration levels of peptide sequences of the protein of interest. The robust and complete estimation of this distribution for as many peptide fragments as possible is instrumental to understanding dynamic protein-level HX behavior. Currently, this data interpretation step still is a bottleneck in the overall HX/MS workflow.
Item Description:Gesehen am 18.04.2023
Physical Description:Online Resource
ISSN:1367-4811
DOI:10.1093/bioinformatics/btq165

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