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Single molecule atomic force microscopy studies of photosensitized singlet oxygen behavior on a DNA origami template

DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can...

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Main Authors: Helmig, Sarah (Author) , Rotaru, Alexandru (Author) , Arian, Dumitru (Author) , Kovbasyuk-Mokhir, Larisa (Author) , Arnbjerg, Jacob (Author) , Ogilby, Peter R. (Author) , Kjems, Jørgen (Author) , Mokhir, Andriy (Author) , Besenbacher, Flemming (Author) , Gothelf, Kurt V. (Author)
Format: Article (Journal)
Language:English
Published: 28 December 2010
In: ACS nano
Year: 2010, Volume: 4, Issue: 12, Pages: 7475-7480
ISSN:1936-086X
DOI:10.1021/nn102701f
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/nn102701f
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Author Notes:Sarah Helmig, Alexandru Rotaru, Dumitru Arian, Larisa Kovbasyuk, Jacob Arnbjerg, Peter R. Ogilby, Jørgen Kjems, Andriy Mokhir, Flemming Besenbacher, and Kurt V. Gothelf
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Summary:DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami.
Item Description:Gesehen am 19.04.2023
Physical Description:Online Resource
ISSN:1936-086X
DOI:10.1021/nn102701f

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